| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCCLELLAND, Amick | SO | Quincy (Sr.) | 2:31.66 | Verified |
| 2 | UPPINGHOUSE, Nataly | FR | Quincy (Sr.) | 2:55.00 | Unverified: Galesburg Girls Invitational |
| 3 | WIGFALL, Riah | SO | Hannibal High School | 3:05.64 | Verified |
| 4 | PLENGE, Irelyn | FR | Clark County High School | 3:05.93 | Verified |
| 5 | BERHORST, Piper | FR | Hannibal High School | NT | Unverified: |
| 6 | KORTE, Lucy | FR | Bowling Green High School | NT | Unverified: |
| 10 | BONVILLIAN, Ava | SO | Hannibal High School | NT |