| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | HARDIN, ISAIAH | SO | Clark County High School | 47.47 | Verified |
| 3 | WHITESIDE, Chance | SO | Hannibal High School | 43.82 | Verified |
| 4 | DAVIS, Cameron | SO | Hannibal High School | 45.75 | Verified |
| 5 | GOGINENI, Sathvik | SO | Quincy (Sr.) | 55.80 | Verified |
| 6 | JAWORSKI, Joshua | SO | Hannibal High School | NT |