| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Quincy (Sr.) | A | O'Hara | Robinson | Nauert | Torres | 47.79 | Unverified: 11th Annual Streak ABC Invite |
| 2 | Bowling Green High School | A | Ream | Buffington | Niemeyer | Oberhaus | 44.68 | Verified |
| 3 | Hannibal High School | A | Odom | Ferreira | Hall | Ferreira | 43.72 | Verified |
| 4 | Mexico High School | A | Dingledine | Washington | Rowan | Potter | 43.73 | Verified |
| 5 | Clark County High School | A | Randle | HARDIN | Rodgers | BURGESS | 44.95 | Verified |