| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MACDONOUGH, Cameron | SO | Quincy (Sr.) | 4:54.56 | Verified |
| 2 | MUNGER, David | SO | Hannibal High School | 4:55.96 | Verified |
| 3 | THOMPSON, Jonas | FR | Hannibal High School | 5:06.04 | Verified |
| 4 | ALLEN, Tanner | FR | Hannibal High School | 5:26.00 | Verified |
| 5 | MATLOCK, Daniel | FR | Bowling Green High School | 5:30.65 | Verified |
| 6 | STEINER, Reed | SO | Quincy (Sr.) | 5:36.28 | Verified |
| 7 | AKRIGHT, Gage | FR | Hannibal High School | 5:39.04 | Verified |
| 8 | NADING, Carter | FR | Mexico High School | 5:41.00 | Verified |
| 9 | MARTIN, Ismael | SO | Hannibal High School | 5:45.99 | Verified |
| 10 | ROACH, Quintin | FR | Clark County High School | 5:58.06 | Verified |
| 11 | LEWIS, KYLE | FR | Mexico High School | 6:11.82 | Verified |
| 12 | THOMPSON, Brayden | SO | Clark County High School | 7:00.95 | Verified |