| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Affton High School | A | Daughtry | Hampton | Horn | Mulat | NT | Unverified: |
| 2 | Kirkwood High School | C | Tubbesing | Howell | Mitchell | Hooker | NT | Verified |
| 3 | Lutheran North High School | A | Bolden | Garner | Carr | Stringfellow | 4:30.35 | Verified |
| 4 | Kirkwood High School | A | Fleming | Wilkes | Canatsey | Dixon | 4:16.10 | Unverified: |
| 5 | Kirkwood High School | B | Wright | Martz | McClure | MacLellan | 4:21.50 | Unverified: |
| 6 | St. Dominic High School | A | Ryan | Zeigler | Robertson | Burle | 4:38.05 | Verified |
| 7 | Parkway North High School | A | Nelson Grimes | Zheleva | Fish | Smith | NT | Unverified: |