| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Kirkwood High School | A | Birmingham | Hickman | Snyder | Stevener | 8:23.50 | Unverified: |
| 2 | Kirkwood High School | B | Larson | Richman | Harrison | Kramer | 8:40.00 | Unverified: |
| 3 | Parkway North High School | A | James | Powell | Tate | Cain | 9:04.53 | Verified |
| 4 | Affton High School | D | NT | Verified | ||||
| 5 | Affton High School | B | Mendenhall | Zeric | Street | Skampo | 9:43.80 | Verified |
| 6 | Affton High School | C | Mooney | 9:43.80 | Verified | |||
| 7 | Affton High School | A | Bethel | Dullovi | Kelly | Lang | 9:43.80 | Verified |
| 8 | St. Dominic High School | B | Fink | Drohr | Steinbruegge | Soval | 9:58.92 | Verified |
| 9 | St. Dominic High School | A | Tracy | Willenbrink | Troesser | Pierce | NT | Verified |
| 10 | Lutheran North High School | A | Gleghorn | Bogan | Coleman | McCollum | 10:07.84 | Verified |
| 11 | Clayton High School | A | Baker | Hoette | Zou | Keller | NT | Unverified: |