| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | COUSINS, Addison | FR | Affton High School | NT | Unverified: |
| 3 | LANG, Vivian | SO | Affton High School | NT | Unverified: |
| 4 | COLEMAN, Angel | SO | Jennings High School | 36.13 | Verified |
| 5 | ZIMMERMAN, Riley | SO | Clayton High School | NT | Unverified: |
| 6 | KABARGINA, Margaret | FR | Clayton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROLLEY, Vonyae | SO | Jennings High School | 33.02 | Verified |
| 2 | LEWIS, Sariah | SO | Jennings High School | 31.33 | Verified |
| 3 | STRICKLAND, Janyah | FR | Lutheran North High School | 30.74 | Verified |
| 4 | TILLMAN, Alaina | SO | Parkway North High School | 30.10 | Verified |
| 5 | MCWILLIAMS, London | JR | Parkway North High School | 30.32 | Verified |
| 6 | MACK, Kirstan | SR | Lutheran North High School | 30.80 | Verified |
| 7 | BASSE, Ellen | SR | St. Dominic High School | 31.71 | Verified |
| 8 | MCFEE, Lashera | FR | Affton High School | 33.96 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HILTON, Christen | SO | Parkway North High School | 28.70 | Verified |
| 2 | GLADNEY, Diamond | SR | Lutheran North High School | 28.34 | Verified |
| 3 | BEAUMONT, Jessie | SR | Kirkwood High School | 27.17 | Verified |
| 4 | SANDERS, Mekhila | JR | Clayton High School | 26.01 | Verified |
| 5 | WILKES, Mariah | JR | Kirkwood High School | 26.76 | Verified |
| 6 | POLLARD, Victoria | SR | St. Dominic High School | 27.60 | Verified |
| 7 | DIXON, Summer | SO | Kirkwood High School | 28.59 | Verified |
| 8 | ZEIGLER, Heidi | FR | St. Dominic High School | 29.24 | Verified |