| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | THOMAS, Camden | JR | Parkway North High School | 2:58.73 | Verified |
| 2 | DULLOVI, Avni | SO | Affton High School | 2:58.87 | Verified |
| 3 | MORALES, Kevin | FR | Affton High School | 2:59.52 | Verified |
| 4 | KELLY, Dylon | SO | Affton High School | 3:02.92 | Verified |
| 5 | MENDENHALL, Logan | FR | Affton High School | 3:07.53 | Verified |
| 6 | SKAMPO, Eldin | SO | Affton High School | 3:09.86 | Verified |
| 7 | STREET, Corey | SO | Affton High School | 3:17.99 | Verified |
| 8 | SOELL, August | FR | Kirkwood High School | NT | Unverified: |
| 9 | LAIRD, John | FR | Kirkwood High School | NT | Unverified: |
| 10 | JAMES, Roderick | FR | Parkway North High School | NT | Unverified: |
| 11 | ZOU, Dante | FR | Clayton High School | NT | Unverified: |
| 12 | BUTE, Brendan | JR | Affton High School | NT | Unverified: |
| 13 | KRONE, Austin | FR | Parkway North High School | NT | Unverified: |
| 14 | POWELL, Kane | FR | Parkway North High School | NT | Unverified: |
| 15 | EARLL, Mason | SO | Affton High School | NT | Unverified: |
| 16 | WOOD, Henry | SO | St. Dominic High School | NT | Unverified: |
| 17 | BETHEL, Weston | FR | Affton High School | NT | Unverified: |
| 18 | BAKER, Luke | JR | Clayton High School | NT | Unverified: |
| 19 | PETTI, Hayden | FR | Affton High School | NT | Unverified: |
| 20 | AVERBECK, Peyton | FR | Affton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOINER, Jack | SR | Kirkwood High School | 2:30.52 | Verified |
| 2 | PRUDE, Jasper | SR | Lutheran North High School | 2:32.31 | Verified |
| 3 | DEPALMA, Nicholas | SR | Kirkwood High School | 2:33.07 | Verified |
| 4 | BARNES, Marrione | FR | Lutheran North High School | 2:34.39 | Verified |
| 5 | MOEHLENBROCK, Bailey | JR | Affton High School | 2:34.62 | Verified |
| 6 | LANG, Connor | SO | Affton High School | 2:36.11 | Verified |
| 7 | BUSBY, Conner | JR | St. Dominic High School | 2:40.17 | Verified |
| 8 | SANER, Bora | JR | Clayton High School | 2:41.57 | Verified |
| 9 | SPERATO, Graham | FR | Kirkwood High School | 2:41.69 | Verified |
| 10 | GRAY, Kellen | FR | St. Dominic High School | 2:41.98 | Verified |
| 11 | SUN, Samuel | JR | Clayton High School | 2:42.45 | Verified |
| 12 | LARSON, Charles | FR | Kirkwood High School | 2:42.84 | Verified |
| 13 | DUBISSON, Thierry | FR | Kirkwood High School | 2:44.59 | Verified |
| 14 | SOVAL, Jacob | JR | St. Dominic High School | 2:45.98 | Verified |
| 15 | ESPE, Bennett | JR | Clayton High School | 2:46.00 | Verified |
| 16 | FINK, James | SO | St. Dominic High School | 2:46.95 | Verified |
| 17 | WEISS, William | FR | Affton High School | 2:47.10 | Verified |
| 18 | DROHR, Garrett | FR | St. Dominic High School | 2:47.66 | Verified |
| 19 | OMEROVIC, Emrah | SR | Affton High School | 2:49.65 | Verified |
| 20 | ZERIC, Dino | JR | Affton High School | 2:50.42 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOHNSON, Sylvester | JR | Lutheran North High School | 2:03.18 | Verified |
| 2 | HICKMAN, Andrew | SR | Kirkwood High School | 2:05.94 | Verified |
| 3 | BIRMINGHAM, Griffin | JR | Kirkwood High School | 2:08.48 | Verified |
| 4 | WILLENBRINK, Tyler | SR | St. Dominic High School | 2:11.88 | Verified |
| 5 | RICHMAN, George | SO | Kirkwood High School | 2:13.08 | Verified |
| 6 | STEVENER, Graham | SO | Kirkwood High School | 2:13.25 | Verified |
| 7 | SNYDER, Joseph | JR | Kirkwood High School | 2:13.41 | Verified |
| 8 | TROESSER, Alex | JR | St. Dominic High School | 2:13.59 | Verified |
| 9 | LOVELACE, Josiah | JR | Affton High School | 2:16.42 | Verified |
| 10 | HOETTE, Charles | SR | Clayton High School | 2:16.97 | Verified |
| 11 | MCDEVITT, Hayden | SR | Parkway North High School | 2:17.45 | Verified |
| 12 | SHIELDS, Alex | JR | Parkway North High School | 2:18.92 | Verified |
| 13 | CAIN, Logan | JR | Parkway North High School | 2:19.15 | Verified |
| 14 | PALMER, Caleb | SR | Lutheran North High School | 2:21.87 | Verified |
| 15 | FERKEL, Peyton | SO | Kirkwood High School | 2:23.31 | Verified |
| 16 | MOONEY, Tyler | SO | Affton High School | 2:25.95 | Verified |
| 17 | TRACY, Alex | SR | St. Dominic High School | 2:27.84 | Verified |
| 18 | PIERCE, Kevin | SO | St. Dominic High School | 2:28.19 | Verified |
| 19 | MANDERNACH, Jackson | JR | Affton High School | 2:28.28 | Verified |
| 20 | GILL, Owen | JR | Parkway North High School | 2:28.95 | Verified |