| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | OSREDKER, Alaina | SO | St. Dominic High School | 2:56.96 | Verified |
| 2 | SCHREIBER, Emma | FR | St. Dominic High School | 2:59.81 | Verified |
| 3 | UPHOFF, Amelia | FR | Parkway North High School | 3:07.08 | Verified |
| 4 | BEUTELMANN, Anna | SO | Clayton High School | 3:08.69 | Verified |
| 5 | BAUMGARTNER, Amelia | FR | Kirkwood High School | 3:08.74 | Verified |
| 6 | DAVIS, Savannah | FR | Parkway North High School | 3:18.03 | Verified |
| 7 | HUFFSTUTTER, Alizabeth | FR | St. Dominic High School | 3:19.23 | Verified |
| 8 | HAMILTON, Sydney | SO | St. Dominic High School | 3:27.93 | Verified |
| 9 | KELL, Andi | FR | St. Dominic High School | 3:37.30 | Verified |
| 10 | DAYAL, Sonali | SR | Clayton High School | 3:38.88 | Verified |
| 11 | WEST, Lily | FR | St. Dominic High School | 3:39.82 | Verified |
| 12 | ENDALKACHEW, Ephrata | SO | Lutheran North High School | 4:06.38 | Verified |
| 13 | BOWMAN, Madeline | SO | St. Dominic High School | 4:16.58 | Verified |
| 14 | HUMPHRIES, Alivia | FR | St. Dominic High School | NT | Unverified: |
| 15 | STRICKLAND, Janyah | FR | Lutheran North High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ERDMANN, Isabel | SR | Clayton High School | 2:21.30 | Verified |
| 2 | DAUGHTRY, Anna | SO | Affton High School | 2:26.96 | Verified |
| 3 | BOLSER, Shelby | SR | Kirkwood High School | 2:34.44 | Verified |
| 4 | WONG, Sophia | SO | Parkway North High School | 2:36.76 | Verified |
| 5 | MCGINNIS, Anna | JR | Kirkwood High School | 2:39.07 | Unverified: Old School/Early Bird |
| 6 | FLEMING, Raven | JR | Kirkwood High School | 2:40.34 | Unverified: Old School/Early Bird |
| 7 | TROESSER, Madison (Maddie) | JR | St. Dominic High School | 2:45.60 | Verified |
| 8 | WIEGMANN, Piper | FR | St. Dominic High School | 2:45.66 | Verified |
| 9 | KLEIN, Katie | SO | Kirkwood High School | 2:46.14 | Verified |
| 10 | BUSBY, Lillian | JR | St. Dominic High School | 2:46.45 | Verified |
| 11 | RISWADKAR, Penelope | JR | Clayton High School | 2:47.28 | Verified |
| 12 | RYAN, Abigail | SR | St. Dominic High School | 2:51.09 | Verified |
| 13 | CHUNG, Rachel | JR | Clayton High School | 2:51.30 | Verified |
| 14 | CAROLINE WELCH, Ella | SO | Clayton High School | 2:55.22 | Verified |
| 15 | CYR, Elise | FR | St. Dominic High School | 2:55.32 | Verified |