| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clayton High School | A | Stern | Sanders | Ramirez Taylor | Miller | NT | Unverified: |
| 4 | Clayton High School | B | Kabargina | Wolpert | Zimmerman | Pittman | NT | Unverified: |
| 5 | Parkway North High School | A | Smith | Nelson Grimes | Omunu | Hilton | NT | Unverified: |
| 6 | Lutheran North High School | A | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Kirkwood High School | D | Kuhn | Carnell | Reithel | Smith | 1:00.50 | Unverified: |
| 2 | Kirkwood High School | B | Taylor | Madison | McClendon | Burgett | 57.74 | Verified |
| 3 | Affton High School | A | Cella | Daniel | Lang | McFee | 57.17 | Verified |
| 4 | St. Dominic High School | A | Pollard | Metherd | Arrowood | Burle | 52.29 | Verified |
| 5 | Kirkwood High School | A | Stevenson | Pride | Beaumont | Bert | 54.10 | Unverified: |
| 6 | Affton High School | B | NT | Verified | ||||
| 7 | Kirkwood High School | C | Savage | Barry | Goodwin | Barry | 59.70 | Unverified: |
| 8 | Kirkwood High School | E | Feltner | McDaniel | Laciny | Motti | NT | Unverified: |