| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Liberty High School | A | Nilson | Giese | Celmer | Bindel | 1:38.64 | Unverified: |
| 4 | Blue Springs South High School | A | Stauffer | Pyle | Burgess | Flowers | 1:36.00 | Unverified: |
| 5 | Blue Springs High School | A | George | Dudley | Davenport | Parker | 1:36.00 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cameron High School | A | Meadows | Gates | Nelson | Garr | 1:35.49 | Verified |
| 2 | Ruskin High School | A | Butler | Smith | Henderson | Calvin | 1:35.03 | Unverified: |
| 3 | North Kansas City High School | A | Anyabolu | Woods-smith | Rodgers | VerDught | 1:34.57 | Verified |
| 4 | Rockhurst High School | A | Brewer | Smith | Ryan | Yarbrough | 1:30.49 | Verified |
| 5 | Grain Valley High School | A | Anderson | Hart | McKinley-Harden | Olah | 1:31.67 | Verified |
| 6 | Fort Osage High School | A | Holloway | Lamb | Scheuring-Holman | Snodgrass | 1:35.02 | Verified |
| 7 | Lee's Summit West High School | A | 1:35.22 | Verified |