| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Liberty High School | A | Bindel | Romero | Lewis | Miner | 1:10.00 | Unverified: |
| 2 | North Kansas City High School | A | Bradley | Still | Matthews | Baehr | 45.46 | Verified |
| 3 | Raymore-Peculiar High School | A | 43.77 | Verified | ||||
| 4 | Blue Springs South High School | A | Al-lgaily | Wyckoff | Wheeler | Greashaber | 43.28 | Verified |
| 5 | Ruskin High School | A | SMITH | Payne Jr | MCKINNEY BYNUM | Young | 43.57 | Verified |
| 6 | Grain Valley High School | A | Bown | Goodwin | Blackmon | Nelson | 44.09 | Verified |
| 7 | Cameron High School | A | Eaton | Shaw | McBride | Lushku | 45.66 | Verified |