| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | North Kansas City High School | A | Sage | Kiliba | Thomas | Williams | 1:00.00 | Unverified: |
| 3 | Grain Valley High School | A | Moore | Mason | Hernandez | Wright | 51.28 | Verified |
| 4 | Raymore-Peculiar High School | A | DNS | Verified | ||||
| 5 | Blue Springs South High School | A | DNS | Verified | ||||
| 6 | Notre Dame de Sion High School | A | Burdge | Carlson | Gromowsky | Saxton | 54.45 | Verified |
| 7 | Liberty High School | A | Vogelaar | Hubbard | Franklin | Waller | DNS | Unverified: |