| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Liberty High School | A | Smith | Hinojos | OBrien | Hurd | 4:50.77 | Unverified: |
| 2 | Cameron High School | A | Jameson | Wenck | McVicker | Robinson | 4:20.58 | Verified |
| 3 | Grain Valley High School | A | Fisher | Reed | Goulden | Mason | 4:18.67 | Verified |
| 4 | Lee's Summit West High School | A | Broesder | Myers-Ray | Carpenter | Wattree | 4:06.21 | Verified |
| 5 | Raymore-Peculiar High School | A | Simmons | Mitchell | Smith | Wade | 4:11.44 | Unverified: |
| 6 | Blue Springs High School | A | Bronson | Sipes | Wild | Wooderson | 4:20.00 | Unverified: |
| 7 | North Kansas City High School | A | Butler | Newton | Toto | Owens | DNS | Unverified: |
| 8 | Notre Dame de Sion High School | A | Traylor | Lind | Carlson | Shelton | 4:51.23 | Verified |