| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | SHINN, Makenzie | SR | Liberty High School | 33.83 | Verified |
| 4 | THORNBURG, Adryelle | FR | Liberty High School | 32.14 | Unverified: |
| 5 | FREDERIQUE, Jaden | JR | North Kansas City High School | 32.48 | Verified |
| 6 | GASKIN, Amaya | FR | North Kansas City High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARLOW, Rebecca | FR | Fort Osage High School | 31.20 | Verified |
| 2 | ZEY, Olivia | FR | Blue Springs High School | 30.74 | Verified |
| 3 | ANDES, Callie | FR | Blue Springs High School | 29.96 | Verified |
| 4 | GOULDEN, Justice | SO | Grain Valley High School | 29.45 | Verified |
| 5 | RUIZ, Izzy | FR | Notre Dame de Sion High School | 29.94 | Unverified: Brutus Hamilton |
| 6 | JOHNSON, Annesta | JR | North Kansas City High School | 30.33 | Verified |
| 7 | DUNN, Kylie | FR | Fort Osage High School | 30.78 | Verified |
| 8 | DELGADO, Xadielys | FR | Blue Springs High School | 31.87 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLAUSEN, Nevaeh | SO | Grain Valley High School | 29.28 | Verified |
| 2 | HICKS, Miori | FR | Blue Springs South High School | 28.67 | Verified |
| 3 | ARCENEAUX, Bailey | JR | Ruskin High School | 28.52 | Verified |
| 4 | EIDSON, Virginia | FR | Blue Springs South High School | 28.29 | Verified |
| 5 | BURRELL, Ayania | SO | Ruskin High School | 28.35 | Verified |
| 6 | DEACON, Caroline | SO | Notre Dame de Sion High School | 28.61 | Verified |
| 7 | WILLIAMS, Rodjanae | JR | Raymore-Peculiar High School | 28.73 | Verified |
| 8 | THOMAS, Paige | SR | Blue Springs South High School | 29.31 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SARIAH, Kamryn | JR | Lee's Summit West High School | 27.20 | Verified |
| 2 | FONTLEROY, Samya | SO | Ruskin High School | 26.75 | Verified |
| 3 | SIMMONS, Kynah | SO | Raymore-Peculiar High School | 26.22 | Verified |
| 4 | SCOTT, Mikah | SR | Lee's Summit West High School | 25.10 | |
| 5 | SHELTON, Emerson | JR | Notre Dame de Sion High School | 26.19 | Verified |
| 6 | BIRMINGHAM, Jaimy | SO | Lee's Summit West High School | 26.23 | Verified |
| 7 | ROGERS, Madison | SR | Grain Valley High School | 26.95 | Verified |
| 8 | YOUNG, Brooklyn | SR | Liberty High School | 28.17 | Verified |