| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | PENN, Deleah | SO | Ruskin High School | NT | Unverified: |
| 3 | MOSES, Micah | FR | Notre Dame de Sion High School | 1:09.74 | Verified |
| 4 | STAUFELDT, Caroline | JR | Liberty High School | 1:02.44 | Verified |
| 5 | DYKE, Amaris | FR | Notre Dame de Sion High School | 1:04.01 | Verified |
| 6 | REED, Morgan | SO | Ruskin High School | 1:10.15 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STARR, Aleecia | JR | Ruskin High School | 58.74 | Verified |
| 2 | WILLIAMS, Aaliyah | SO | Blue Springs High School | 56.84 | Verified |
| 3 | HEINRICH, Samantha | SR | Grain Valley High School | 56.00 | Unverified: |
| 4 | WOOD, Miller | FR | Lee's Summit West High School | 54.64 | Verified |
| 5 | ZORN, Lacey | FR | Fort Osage High School | 55.50 | Verified |
| 6 | HUMPHREY-MENDEZ, Esmeralda | SO | Blue Springs South High School | 56.43 | Verified |
| 7 | HOWARD, Brielle | FR | Fort Osage High School | 58.35 | Verified |
| 8 | MERGANI, Shafaq | JR | North Kansas City High School | 1:01.50 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REED, Aspen | FR | Grain Valley High School | 53.00 | Unverified: |
| 2 | BREWER, Justice | JR | Cameron High School | 50.94 | Verified |
| 3 | WENCK, Claire | SR | Cameron High School | 49.69 | Verified |
| 4 | FURNELL, Sydney | SO | Lee's Summit West High School | 48.95 | Verified |
| 5 | WEST, Izabella | JR | Grain Valley High School | 49.00 | Unverified: |
| 6 | HEIDBREDER, Haley | JR | Blue Springs South High School | 50.60 | Verified |
| 7 | MITCHELL, Jadynne | SO | Raymore-Peculiar High School | 52.34 | Verified |
| 8 | NUNN, Rhamaya | SO | Blue Springs South High School | 54.52 | Verified |