| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | WEDEKEMPER, Lily | FR | Centralia (Christ Our Rock Lutheran) | 15.54 | |
| 3 | CAIN, Caroline | FR | Alton (Marquette) | 14.11 | |
| 4 | OHM, Charlotte | SO | Edwardsville (Metro-East Lutheran) | 14.24 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DAVENPORT, Karly | FR | Alton (Marquette) | 13.58 | |
| 2 | RANGE, Mia | SO | Glen Carbon (Father McGivney) | 13.41 | |
| 3 | GILBERTSON, Lilly | SO | Glen Carbon (Father McGivney) | 12.74 | |
| 4 | ADUBATO, Bianca | SR | Centralia (Christ Our Rock Lutheran) | 13.00 | |
| 5 | WATKINS, Zoey | FR | Bunker Hill | 13.47 | |
| 6 | SHERFY, Claire | JR | Edwardsville (Metro-East Lutheran) | 13.89 |