| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Glen Carbon (Father McGivney) | A | Biermann | Joellenbeck | Shearer | Moore | 49.25 | |
| 3 | Edwardsville (Metro-East Lutheran) | A | Fedder | Burnett | Holmes | Kohlmiller | 46.00 | |
| 4 | Maryville (M. Christian) | A | Malone | Ury | King | King | 46.28 |