| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ADUBATO, Bianca | SR | Centralia (Christ Our Rock Lutheran) | NM | |
| 2 | HARRIS, Isabella | SO | Glen Carbon (Father McGivney) | 25' 7" | |
| 3 | SHERFY, Claire | JR | Edwardsville (Metro-East Lutheran) | 29' 7" | |
| 4 | SECHREST, Grace | SR | Alton (Marquette) | 31' 8" | |
| 5 | HENTRICH, Sammy | SR | Alton (Marquette) | 33' 8" |