| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Glen Carbon (Father McGivney) | A | Terrell | Ivnik | Windorff | Pinkston | 59.00 | |
| 3 | Edwardsville (Metro-East Lutheran) | A | Ohm | Koch | Hughes | Sherfy | 54.80 | |
| 4 | Alton (Marquette) | A | Cain | Sechrest | Bryson | Bryson | 55.40 |