| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Centralia (Christ Our Rock Lutheran) | A | Koch | Lu | Lyons | Gregory | 3:54.50 | |
| 2 | Edwardsville (Metro-East Lutheran) | A | Fields | Holmes | Pryor | Schlessinger | 3:49.12 | |
| 3 | Alton (Marquette) | A | Fears | Nash | Kline | Rochester | DNS | |
| 4 | Maryville (M. Christian) | A | Ury | Abert | Jacob | Gillihan | 3:41.40 | |
| 5 | Glen Carbon (Father McGivney) | A | Ahring | Moore | Kohlberg | Biermann | 3:52.00 |