| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BAKER, Jesse | FR | Bunker Hill | 26.34 | |
| 3 | CHERNIANSKII, Mark | JR | Edwardsville (Metro-East Lutheran) | 25.78 | |
| 4 | NASH, Braden | FR | Alton (Marquette) | 26.14 | |
| 5 | MORRIS, Alijah | FR | Centralia (Christ Our Rock Lutheran) | 27.11 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LYONS, Isaiah | SO | Centralia (Christ Our Rock Lutheran) | 24.91 | |
| 2 | MALONE, Gabe | SR | Maryville (M. Christian) | 23.36 | |
| 3 | HUBER, Jacob | SR | Glen Carbon (Father McGivney) | 22.90 | |
| 4 | KING, Kai | SR | Maryville (M. Christian) | 23.11 | |
| 5 | FEDDER, Charles | JR | Edwardsville (Metro-East Lutheran) | 23.84 | |
| 6 | ROCHESTER, Jaden | JR | Alton (Marquette) | 24.94 |