| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ERTEL, Gretchen | SO | Glen Carbon (Father McGivney) | 36.00 | |
| 3 | CAIN, Caroline | FR | Alton (Marquette) | 30.14 | |
| 4 | HUGHES, Emily | JR | Edwardsville (Metro-East Lutheran) | 31.80 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | OHM, Charlotte | SO | Edwardsville (Metro-East Lutheran) | 29.61 | |
| 2 | WATKINS, Zoey | FR | Bunker Hill | 28.84 | |
| 3 | GILBERTSON, Lilly | SO | Glen Carbon (Father McGivney) | 26.13 | |
| 4 | HENTRICH, Sammy | SR | Alton (Marquette) | 26.84 | |
| 5 | ADUBATO, Bianca | SR | Centralia (Christ Our Rock Lutheran) | 29.00 |