| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ROCHESTER, Jaden | JR | Alton (Marquette) | NT | 54.1 |
| 3 | GILLIHAN, James | SR | Maryville (M. Christian) | 55.84 | |
| 4 | FIELDS, Josh | JR | Edwardsville (Metro-East Lutheran) | 56.16 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LU, Yilin | SR | Centralia (Christ Our Rock Lutheran) | 55.67 | |
| 2 | FEARS, Justin | JR | Alton (Marquette) | 54.65 | |
| 3 | HUBER, Jacob | SR | Glen Carbon (Father McGivney) | 51.13 | |
| 4 | URY, Jared | JR | Maryville (M. Christian) | 52.50 | |
| 5 | LYONS, Isaiah | SO | Centralia (Christ Our Rock Lutheran) | 55.34 | |
| 6 | PRYOR, Carter | JR | Edwardsville (Metro-East Lutheran) | 55.79 |