| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Glen Carbon (Father McGivney) | A | Biermann | Shearer | Joellenbeck | Moore | 1:43.00 | |
| 3 | Edwardsville (Metro-East Lutheran) | A | Schlessinger | Burnett | Holmes | Fedder | 1:37.19 | |
| 4 | Alton (Marquette) | A | Nash | Kline | Fears | Rochester | 1:38.00 | |
| 5 | Maryville (M. Christian) | A | Malone | Coulby | Paul | Abert | 1:48.17 |