| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GILLIHAN, James | SR | Maryville (M. Christian) | 2:05.55 | |
| 2 | AHRING, Tyler | SO | Glen Carbon (Father McGivney) | 2:07.85 | |
| 3 | JACOB, Noah | FR | Maryville (M. Christian) | 2:12.98 | |
| 4 | BOEVING, Liam | SO | Glen Carbon (Father McGivney) | 2:14.76 | |
| 5 | BROEKEMEIER, Adam | JR | Edwardsville (Metro-East Lutheran) | 2:15.01 | |
| 6 | KLINE, Daniel | JR | Alton (Marquette) | 2:15.87 | |
| 7 | STAAKE, Nathan | JR | Edwardsville (Metro-East Lutheran) | 2:33.30 | |
| 8 | LYONS, Liagel | FR | Centralia (Christ Our Rock Lutheran) | 2:42.66 |