| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HATLEY, Kaitlyn | JR | Glen Carbon (Father McGivney) | 2:21.54 | |
| 2 | CUMMINS, Jane | SO | Glen Carbon (Father McGivney) | 2:29.56 | |
| 3 | MCKOWEN, Sarah | JR | Centralia (Christ Our Rock Lutheran) | 2:29.75 | |
| 4 | CERTA, Ava | JR | Alton (Marquette) | 2:37.71 | |
| 5 | DONALD, Mia | FR | Maryville (M. Christian) | 2:55.03 | |
| 6 | JACOB, Isabel | FR | Maryville (M. Christian) | 3:06.81 | |
| 7 | ECCLES, Cassidy | SO | Alton (Marquette) | 3:08.96 | |
| 8 | BRAMMEIER, Rylee | SO | Centralia (Christ Our Rock Lutheran) | NT | |
| 9 | VICKREY, Kailey | SR | Alton (Marquette) | NT | 2:48.00 |