| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TURNBOUGH, Audrey | JR | Steelville High School | 5:58.25 | Verified |
| 2 | MCDANIEL, Tajel | SO | Belle High School | 6:21.94 | Verified |
| 3 | BASHAM, Cadynce | FR | Cuba High School | 6:24.71 | Verified |
| 4 | COLLINSON, Aleah | JR | Steelville High School | 6:26.70 | Verified |
| 5 | HARDCASTLE, Patricia | FR | Cuba High School | 6:43.11 | Verified |
| 6 | COLLINSON, Lydia | FR | Steelville High School | 7:08.84 | Verified |