| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cuba High School | A | Dake | Laney | DeClue | Bonney | 1:41.46 | Verified |
| 4 | Steelville High School | A | Lorraine | Midyett | Settles | Killeen | 1:38.02 | Verified |
| 5 | Vienna High School | A | Kleffner | Schwartze | Ewers | Hayes | 1:41.43 | Verified |
| 6 | Belle High School | A | Weigel | Gromowski | Valley | Smith | 1:45.96 | Verified |