| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Steelville High School | A | Brooks | Wishon | Bailey | Diaz | 2:00.44 | Verified |
| 4 | Vienna High School | A | Schulte | Rosentreter | Schell | Reeves | 1:56.78 | Verified |
| 5 | Bourbon High School | A | Clonts | Enke | Enke | King | 1:57.28 | Verified |
| 6 | Cuba High School | A | Black | Moreland | Martellaro | Lunsford | 2:04.58 | Verified |