| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cuba High School | A | Dake | Halbert | Hinojosa | Volner | 3:54.11 | Verified |
| 4 | Steelville High School | A | Midyett | Harris | Diaz | Brown | 3:35.71 | Verified |
| 5 | Vienna High School | A | Wilkinson | Ewers | Wulff | Kilmer | 3:47.31 | Verified |