| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cuba High School | A | Black | Hardcastle | Viramontes | Weber | 4:55.21 | Verified |
| 4 | Vienna High School | A | Novak | Novak | Wieberg | Reeves | 4:30.16 | Verified |
| 5 | Steelville High School | A | Diaz | Pryor | Kreitner | Pryor | 4:38.13 | Verified |