| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Conner | SR | Steelville High School | 1:59.19 | Verified |
| 2 | WILKINSON, Duncan | JR | Vienna High School | 2:08.73 | Verified |
| 4 | MORELAND, Austin | JR | Steelville High School | 2:29.36 | Verified |
| 6 | SMITH, Jeremy | FR | Cuba High School | 2:34.21 | Verified |
| 7 | DOUGLAS, Thomas | FR | Vienna High School | 2:37.21 | Verified |
| 8 | HARRIS, Tyson | JR | Steelville High School | 2:41.14 | Verified |
| 9 | HOEHNE, Adrien | JR | Belle High School | 3:02.73 | Verified |