| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Vienna High School | A | Kleffner | Schwartze | Hayes | Wulff | 49.67 | Verified |
| 4 | Cuba High School | A | Laney | Halbert | Volner | Hinojosa | 46.82 | Verified |
| 5 | Steelville High School | A | Killeen | Midyett | Settles | Brown | 48.46 | Verified |
| 6 | Belle High School | A | Bailey | Schmid | Butler | Gromowski | NT | Verified |