| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cuba High School | A | Dunham | Moreland | Viramontes | Weber | 57.73 | Verified |
| 4 | Steelville High School | A | Kelley | Kreitner | Pryor | Eilert | 55.57 | Verified |
| 5 | Vienna High School | A | Rosentreter | Novak | Novak | Murphy | 56.42 | Verified |
| 6 | Viburnum High School | A | Pyatt | Halbert | Conaway | Wigger | 1:00.07 | Verified |