| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Notre Dame (Cape Girardeau) High | A | Neumann | Le | Pfeifer | Eftink | NT | Unverified: |
| 4 | Ewing Marion Kauffman School | A | Taylor | Johnson | Brock | Coffee | NT | Unverified: |
| 5 | Central (Cape Girardeau) High Sc | B | Sheridan | Taylor | Williams | Newsom | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Dexter High School | A | Farmer | Bryant | Wirz | Hill | NT | Unverified: |
| 3 | Herculaneum High School | B | Meyers | Meyers | Gallina | Luther | 2:15.00 | Unverified: |
| 5 | Jackson High School | B | Settle | Gross | Craigmiles | Geiser | 1:42.00 | Unverified: |
| 6 | Herculaneum High School | A | McCullough | Hawkins | Petri | Cox | 2:20.00 | Unverified: |
| 7 | Ste. Genevieve High School | A | Amschler | Vogt | McBride | McBride | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Scott City High School | A | 1:38.00 | Unverified: | ||||
| 2 | Caruthersville High School | A | Motton | Rodgers | Hunter | Alexander | 1:36.07 | Unverified: |
| 4 | Festus High School | A | 1:33.13 | Unverified: | ||||
| 5 | Central (Cape Girardeau) High Sc | A | Brooks-Davis | Fullenwider | Dixon | Edwards | 1:35.60 | Unverified: |
| 6 | DeSoto High School | A | Bourn | Missey | Smith | Pirtle | 1:36.00 | Unverified: 47 |
| 7 | Jackson High School | A | Boyd | CAIN | Alspaugh | Reagan | 1:36.50 | Unverified: |
| 8 | DeSoto High School | B | Akers | Flack | Patterson | Thebeau | 1:40.00 | Unverified: 50 |