| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Notre Dame (Cape Girardeau) High | A | Eftink | Pfeifer | DeJournett | Stiegemeyer | NT | Unverified: |
| 2 | Central (Cape Girardeau) High Sc | B | Murray | Eanes | Rusten | Dixon | NT | Unverified: |
| 3 | Saxony Lutheran High School | A | Chambless | Mueller | Richey | Mayhew | NT | Unverified: |
| 4 | Jackson High School | B | Metzger | Hicks | King | Goodson | 4:02.00 | Unverified: |
| 5 | Scott City High School | B | NT | Unverified: | ||||
| 6 | Kelly High School | A | Graham | Appleton | Burleson | Genereau | NT | Unverified: |
| 8 | Ste. Genevieve High School | A | Butcher | Smith | Stafford | Amschler | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jackson High School | A | Boyd | Hutson | COFFEY | Niedbalski | 3:50.00 | Unverified: |
| 2 | Scott City High School | A | 3:47.00 | Unverified: | ||||
| 3 | Central (Cape Girardeau) High Sc | A | Clemons | Allen | Seabaugh | Edwards | 3:40.00 | Unverified: |
| 4 | Festus High School | A | 3:32.76 | Unverified: | ||||
| 5 | DeSoto High School | A | Roland | Patterson | Missey | Bourn | 3:40.00 | Unverified: |
| 6 | Festus High School | B | NT | Unverified: | ||||
| 7 | Dexter High School | A | Kyle | Farmer | Hill | 3:48.00 | Unverified: | |
| 8 | Caruthersville High School | A | McLeod | Hopkin | Sargent | Hunter | 4:00.00 | Unverified: |