| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | THIER, Ella | SR | Rockwood Summit High School | 11:51.24 | Verified |
| 3 | LEFTWICH, Ava | SR | Festus High School | 12:20.07 | Verified |
| 4 | WILSON, Mara | SR | Jackson High School | 12:22.78 | Verified |
| 5 | MARRIOTT, Emily | SO | Central (Cape Girardeau) High Sc | 12:39.11 | Verified |
| 6 | THORNBORROW, Jeannie | JR | Festus High School | 12:40.45 | Verified |
| 7 | BARKS, Annika | SO | Jackson High School | 12:42.00 | Unverified: |
| 8 | PIGG, JAILEY | JR | DeSoto High School | 12:58.21 | Unverified: |
| 9 | ROTH, Myra | SO | Saxony Lutheran High School | 13:25.08 | Verified |
| 10 | MULLINS, Sophia | SO | DeSoto High School | 14:55.50 | Unverified: |
| 11 | DEHNE, Eden | SR | Saxony Lutheran High School | 16:06.60 | Verified |
| 12 | BROWN, Mia | JR | Rockwood Summit High School | NT | Unverified: |
| 13 | COY, Lily | FR | Notre Dame (Cape Girardeau) High | NT | Unverified: |