| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BIRD, Stormierayn | FR | Dexter High School | NT | Unverified: |
| 4 | BUTTROM, Jamya | FR | Caruthersville High School | 59.00 | Unverified: |
| 5 | HALEY, Abigail | SO | Saxony Lutheran High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HARMON, Kailynn | SO | Ste. Genevieve High School | 54.69 | Verified |
| 2 | FARRAR, Mya | SO | Central (Cape Girardeau) High Sc | 53.13 | Verified |
| 3 | HALIBURTON, Sydni | JR | Malden High School | 52.50 | Unverified: |
| 4 | HOWARD, AUTUMN | SO | Jackson High School | 51.97 | Verified |
| 5 | PRUITT, Allysa | SR | Scott City High School | 52.00 | Unverified: |
| 6 | CARR, Lily | SO | Notre Dame (Cape Girardeau) High | 52.54 | Verified |
| 7 | VIPPERMAN, Mikalyn | SO | Malden High School | 54.20 | Unverified: |
| 8 | BROTHERTON, Lily | FR | Notre Dame (Cape Girardeau) High | 55.25 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BIEHLE, Alexis | SR | Festus High School | 51.51 | Unverified: |
| 2 | METZGER, Gracie | SR | Jackson High School | 51.35 | Verified |
| 3 | HUCK, Kayden | SO | Ste. Genevieve High School | 50.29 | Verified |
| 4 | TURNBO, Allison | JR | Dexter High School | 50.13 | Verified |
| 5 | DOCKERY, Taler | JR | Central (Cape Girardeau) High Sc | 50.20 | Verified |
| 6 | REEVES, Eddyson | JR | Herculaneum High School | 51.12 | Verified |
| 7 | HAWKINS, Jessica | FR | Festus High School | 51.51 | Unverified: |
| 8 | SHELTON, Lila | SR | Rockwood Summit High School | 51.73 | Verified |