| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | CLAY, Shamara | SO | Caruthersville High School | NT | Unverified: |
| 4 | LANDGRAF, Madison | FR | Saxony Lutheran High School | NT | Unverified: |
| 5 | ASHER, Kiaira | JR | Ste. Genevieve High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HENNEMANN, Anna | SR | Notre Dame (Cape Girardeau) High | NT | Unverified: |
| 2 | ROWE, Kennedi | FR | Ewing Marion Kauffman School | NT | Unverified: |
| 3 | ESSNER, Sydney | FR | Notre Dame (Cape Girardeau) High | NT | Unverified: |
| 4 | KILBY, Carlee | FR | Scott City High School | NT | Unverified: |
| 5 | LEE, Ebony | SR | Caruthersville High School | NT | Unverified: |
| 6 | BARRETT, Emily | JR | Dexter High School | NT | Unverified: |
| 7 | FIGUEROA, Lena | FR | Kelly High School | NT | Unverified: |
| 8 | FORD, Evie | FR | Saxony Lutheran High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BARKS, Emily | FR | Jackson High School | 1:15.00 | Unverified: |
| 2 | BANDERMAN, Sara | SR | Festus High School | 1:12.12 | Unverified: |
| 3 | KOEHLER, Avery | JR | Central (Cape Girardeau) High Sc | 1:08.07 | Verified |
| 4 | ROWE, Aniyah | SO | Ewing Marion Kauffman School | 1:07.84 | Verified |
| 5 | NEA, Meredith | FR | Dexter High School | 1:08.00 | Unverified: |
| 6 | KLUMP, Olivia | SO | Ste. Genevieve High School | 1:09.43 | Verified |
| 7 | WINKLER, TATUM | SO | Rockwood Summit High School | 1:14.08 | Verified |
| 8 | BURGE, Kaylynn | FR | Malden High School | 1:16.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MOORE, Camila | SR | Herculaneum High School | 1:06.67 | Verified |
| 2 | JACKSON, Barrett | JR | Rockwood Summit High School | 1:05.98 | Verified |
| 3 | PERRY, Presley | SR | Festus High School | 1:04.26 | Verified |
| 4 | MACKINS, Amiyah | SO | Central (Cape Girardeau) High Sc | 1:00.71 | Verified |
| 5 | CASTILLO-ORELLANA, Maria | SR | Herculaneum High School | 1:03.89 | Verified |
| 6 | GOETZ, Beckley | FR | Jackson High School | 1:05.00 | Unverified: |
| 7 | CRADY, Autymn | SO | DeSoto High School | 1:06.00 | Unverified: |
| 8 | MITKOS, Dominique | SR | DeSoto High School | 1:07.00 | Unverified: |