| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Francis Howell North High School | A | Minter | Ingracia | Kelly | Allen | 1:40.77 | Verified |
| 4 | St. Charles High School | A | Puati | Lopez | Martinez | Wiggs | 1:36.42 | Verified |
| 5 | Liberty (Wentzville) High School | A | Holderfield | Kargacin | Jefferson | Wilkes | 1:37.94 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Union High School | A | Burke | Swoboda | Birke | Pickard | 1:33.86 | Unverified: Washington NIX Relays 2023 |
| 3 | Francis Howell High School | A | 1:31.65 | Verified | ||||
| 4 | Rock Bridge High School | A | Seamon | Bailey | Holt | Hood | 1:28.56 | Unverified: |
| 5 | Ft. Zumwalt West High School | A | DQ | Verified | ||||
| 6 | Troy Buchanan High School | A | Freeman | Hopmann | Keene | Moore | 1:32.00 | Verified |
| 7 | Francis Howell Central High Scho | A | Smith | Page | Payton | Thornton | 1:36.18 | Verified |