| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Charles High School | A | Oetting | Hollrah | Skillington | O'Keeffe | 4:34.27 | Verified |
| 4 | Union High School | A | Brake | Bockhorst | Spiller | Ahner | 4:30.44 | Verified |
| 5 | Francis Howell Central High Scho | A | Belter | McDevitt | Halterman | Myers | DNS | Unverified: FZN invite |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ft. Zumwalt West High School | A | Rider | McDaniel | Maupin | Anderson | 4:21.08 | Verified |
| 3 | Francis Howell High School | A | 4:18.19 | Verified | ||||
| 4 | Rock Bridge High School | A | Parsons | Miller | Crane | Ford | 4:04.66 | Verified |
| 5 | Washington High School | A | Von Behren | James | Keiser | Gilliatt | 4:18.17 | Verified |
| 6 | Troy Buchanan High School | A | Peters | Arndt | Lewis | Ellison | 4:20.40 | Verified |
| 7 | Liberty (Wentzville) High School | A | Omara | Douglas | Kalbac | Torbit | 4:24.03 | Verified |