| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HESTER, Hallee | SO | St. Charles High School | 33.04 | Verified |
| 4 | WALKER-BLACKMON, Saniya | JR | Francis Howell North High School | 32.00 | Verified |
| 5 | WALTERS, Jade | FR | Francis Howell North High School | 32.22 | Verified |
| 6 | WILLETT, Angelica | SR | Francis Howell High School | 38.84 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TZUN-PEREZ, Alison | SO | Ft. Zumwalt West High School | 29.26 | Verified |
| 2 | RIDER, Lyla | SR | Ft. Zumwalt West High School | 28.75 | Verified |
| 3 | MONKMAN, Camren | SO | Union High School | 28.60 | Unverified: |
| 4 | MHANDU, Mariah | JR | Liberty (Wentzville) High School | 28.12 | Verified |
| 5 | WILLIAMS, Kristian | FR | Francis Howell Central High Scho | 28.58 | Verified |
| 6 | HUNOLT, Macie | JR | Troy Buchanan High School | 28.64 | Verified |
| 7 | LEWIS, Tanise | SO | Francis Howell High School | 28.79 | Verified |
| 8 | KULMA, Charlotte | FR | St. Charles High School | 29.83 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SIDWELL, Marissa | JR | Francis Howell High School | 27.97 | Verified |
| 2 | GERRISH, Addyelin | SO | Rock Bridge High School | 27.63 | Verified |
| 3 | MCGEE, Asiyanna | FR | Washington High School | 27.31 | Verified |
| 4 | PETERS, Reagan | FR | Troy Buchanan High School | 26.30 | Verified |
| 5 | ANTHONY, Tijaria | SO | Francis Howell Central High Scho | 26.67 | Verified |
| 6 | GREEN, Leilani | JR | Liberty (Wentzville) High School | 27.36 | Verified |
| 7 | BOCKHORST, Kirsten | SR | Union High School | 27.70 | Verified |
| 8 | FEDENIA, Aida | FR | Rock Bridge High School | 28.00 | Verified |