| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | THORNTON, Don | FR | Francis Howell Central High Scho | 46.54 | Verified |
| 4 | CAMERON, Isaias | FR | Ft. Zumwalt West High School | 45.99 | Verified |
| 5 | WASHINGTON, Darion | FR | Union High School | 46.41 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ORMSBY, James | JR | Liberty (Wentzville) High School | 45.58 | Verified |
| 3 | BLACK, Issac | FR | St. Charles High School | 44.43 | Verified |
| 4 | ALLEN, Jordan | SR | Francis Howell North High School | 44.06 | Verified |
| 5 | DOWDY, Gilbren | SR | Francis Howell North High School | 44.13 | Verified |
| 6 | SUTER, Adam | JR | Ft. Zumwalt West High School | 45.44 | Verified |
| 7 | WARNER, James | JR | St. Charles High School | 45.70 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | EDWARDS, Harrison | SR | Troy Buchanan High School | 43.15 | Verified |
| 2 | MAUCK, Matthew | SO | Liberty (Wentzville) High School | 42.62 | Verified |
| 3 | SWOBODA, Evan | SR | Union High School | 41.57 | Verified |
| 4 | LARUE, Lucas | JR | Troy Buchanan High School | 41.38 | Verified |
| 5 | STAHL, Thomas | SR | Washington High School | 41.42 | Verified |
| 6 | PEARSON, Jaylen | SR | Francis Howell High School | 42.14 | Verified |
| 7 | WITT, Curtiss | FR | Francis Howell High School | 42.79 | Verified |
| 8 | WEBB, Daniel | SR | Rock Bridge High School | 43.33 | Verified |