| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | TORBIT, Laney | JR | Liberty (Wentzville) High School | 1:09.10 | Verified |
| 4 | GIETL, Olivia | FR | Francis Howell North High School | 1:06.77 | Verified |
| 5 | LARAMIE, Lillian | FR | Ft. Zumwalt West High School | 1:08.62 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LONG, Teriyah | JR | St. Charles High School | 1:06.54 | Verified |
| 2 | AHNER, Jada | SO | Union High School | 1:06.27 | Verified |
| 3 | HEAD, Katelyn | FR | Francis Howell North High School | 1:05.78 | Verified |
| 4 | BRAKE, Kelsey | JR | Union High School | 1:05.04 | Verified |
| 5 | LEWIS, Kalea | FR | Troy Buchanan High School | 1:05.18 | Verified |
| 6 | HALTERMAN, Hannah | SO | Francis Howell Central High Scho | 1:06.03 | Verified |
| 7 | GARCIA, Rebecca | FR | Ft. Zumwalt West High School | 1:06.39 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MORAN, Sarah | JR | Francis Howell High School | 1:03.94 | Verified |
| 2 | GREEN, Leilani | JR | Liberty (Wentzville) High School | 1:02.47 | Verified |
| 3 | VON BEHREN, Isabella | SO | Washington High School | 1:01.47 | Verified |
| 4 | FORD, Carolyn | SR | Rock Bridge High School | 1:00.40 | Verified |
| 5 | GILLIATT, Abigail | JR | Washington High School | 1:00.43 | Verified |
| 6 | MILLER, Lauren | SO | Rock Bridge High School | 1:01.64 | Verified |
| 7 | GRIER, Lucy | SR | Francis Howell High School | 1:03.25 | Verified |
| 8 | BELTER, Sydney | SO | Francis Howell Central High Scho | 1:04.63 | Verified |