| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Union High School | A | Sinclair | Duffer | Frazier | Pope | 46.01 | Verified |
| 4 | St. Charles High School | A | Lopez | Murphy | Richard | Mbroh | 45.75 | Verified |
| 5 | Francis Howell Central High Scho | A | Smith | Page | Thornton | Payton | 45.84 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Francis Howell North High School | A | Dowdy | Allen | Brooks | Kelly | 44.97 | Verified |
| 3 | Ft. Zumwalt West High School | A | 44.44 | Verified | ||||
| 4 | Rock Bridge High School | A | Taylor | TRUITT | Seamon | Hood | 42.63 | Verified |
| 5 | Troy Buchanan High School | A | Freeman | Hopmann | Hall | Moore | 43.11 | Verified |
| 6 | Francis Howell High School | A | 44.62 | Verified | ||||
| 7 | Liberty (Wentzville) High School | A | Galati | Jefferson | Williams | Wilkes | 45.61 | Verified |