| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Francis Howell Central High Scho | A | Thornton | Payton | Gil-Farias | Bottini | 1:42.00 | Unverified: |
| 4 | Ft. Zumwalt South High School | A | McCray | Anzaldua Campos | Godefroid | Jaeger | 1:40.58 | Verified |
| 5 | Timberland High School | A | Volkman | Brewer | Jones | Barnes | DQ | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Hazelwood Central High School | A | George | Gunn | Bright | Burns | 1:35.97 | Unverified: Mike Rodgers Invitational |
| 3 | Ft. Zumwalt North High School | A | Hamilton | Noel | Hinkle | Pirrung | DQ | Verified |
| 4 | Riverview Gardens High School | A | McClain | Simmons | Rimmer | Walker | 1:30.00 | Verified |
| 5 | Francis Howell High School | A | 1:31.87 | Verified | ||||
| 6 | North Point High School | A | Gregory | Robinson | Robinson | Washington | 1:34.84 | Verified |
| 7 | St. Charles High School | A | Alcantara | Kelley | Sanders | Fernandez | NT | Verified |