| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Timberland High School | A | Kiner | Diaz | Climer | Stanley | 2:05.00 | Unverified: |
| 4 | Ft. Zumwalt North High School | A | Bredenkamp | Very | Desai | Taylor | 1:58.71 | Unverified: |
| 5 | Ft. Zumwalt South High School | A | Lane | Ritter | Watson | Hendrix | 2:03.27 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Riverview Gardens High School | A | Wortham | Lewis | Robinson | Davis | 1:55.00 | Unverified: |
| 3 | Francis Howell High School | A | Lewis | Ryan | Yost | Barr | 1:53.00 | Unverified: |
| 4 | Francis Howell Central High Scho | A | Sebree | Clark | Ragle | Williams | 1:51.81 | Verified |
| 5 | Hazelwood Central High School | A | Swanson | Scruggs | White | McCanery | 1:52.47 | Unverified: |
| 6 | St. Charles High School | A | Murillo Vallejo | Crossland | Ming | Vargas | NT | Verified |
| 7 | North Point High School | A | Abunaw | Potter | Cook | Wasser | 1:58.00 | Unverified: Timberland Wolfpack Invite |