| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BAGBY, D'Monta | FR | St. Charles High School | NT | Unverified: |
| 4 | MCCRAY, Nygil | FR | Ft. Zumwalt South High School | NT | Unverified: |
| 5 | KATAMBWA, Mulunda | FR | Timberland High School | NT | Unverified: |
| 6 | BURNETT, Delrin | SO | Ft. Zumwalt South High School | 1:04.00 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MCGHEE, Jalen | SO | Riverview Gardens High School | 51.74 | Verified |
| 3 | BOEHM, Colby | SO | St. Charles High School | 50.50 | Verified |
| 4 | JONES, Emanuel | SO | Ft. Zumwalt North High School | 49.06 | Verified |
| 5 | NALLEY, David (Tre) | SO | North Point High School | 50.40 | Verified |
| 6 | WILSON, Joseph | JR | North Point High School | 50.73 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LAWSON, Dane | SO | North Point High School | 47.30 | Verified |
| 2 | COOPER, Jaimen | FR | Francis Howell High School | 45.96 | Verified |
| 3 | WILLIAMS, Reid | SO | Francis Howell High School | 44.68 | Verified |
| 4 | FOREMAN, Tomas | SO | Francis Howell High School | 44.23 | Verified |
| 5 | SANDERS, Lynden | JR | Ft. Zumwalt North High School | 44.61 | Verified |
| 6 | HOLLINS, James | JR | Riverview Gardens High School | 45.24 | Verified |
| 7 | SHELTON, Jerrion | JR | Riverview Gardens High School | 47.25 | Verified |
| 8 | BOTTINI, Joey | SO | Francis Howell Central High Scho | 47.64 | Verified |