| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HANEY, Ashley | FR | North Point High School | NT | Unverified: |
| 4 | KATAMBWA, Christel | SO | Ft. Zumwalt South High School | 1:03.60 | Verified |
| 5 | ALGAS, Denise | FR | Ft. Zumwalt South High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CARAFIOL, Anna | SO | Francis Howell High School | 1:02.98 | Verified |
| 2 | TUCKER, Mallory | SO | Francis Howell High School | 59.87 | Verified |
| 3 | BROWER, Ally | JR | Francis Howell Central High Scho | 57.56 | Verified |
| 4 | HERNDON, Divine | SO | Riverview Gardens High School | 56.84 | Verified |
| 5 | HARRIS, Kayla | SO | Hazelwood Central High School | 57.16 | Verified |
| 6 | RINGWALD, Kayla | JR | North Point High School | 58.54 | Verified |
| 7 | FALLER, Ashley | SO | Francis Howell High School | 1:00.58 | Verified |
| 8 | SANCHEZ, Layla | SO | Francis Howell Central High Scho | 1:03.22 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SWAFFORD, Bonnie | SO | North Point High School | 55.46 | Verified |
| 2 | MCFOWLAND, Pamela | SO | Riverview Gardens High School | 54.89 | Verified |
| 3 | PUATI, Cecile | FR | St. Charles High School | 53.47 | Verified |
| 4 | REECE, Na'Kyia | JR | Hazelwood Central High School | 52.66 | Verified |
| 5 | MADISON, Jamaria | JR | Hazelwood Central High School | 53.40 | Unverified: |
| 6 | PAINTER, Sam | FR | Francis Howell Central High Scho | 54.74 | Verified |
| 7 | SKILLINGTON, Kierra | FR | St. Charles High School | 54.95 | Verified |
| 8 | REESE, Makayla | JR | Ft. Zumwalt South High School | 55.59 | Verified |